1.
- All the individuals on the team are focusing more on being able to complete the main lab procedures independently
- Personally, I have been taking on more responsibility the longer I have been on the team. Currently, our lead senior in the lab, Maria Lancia, is certifying me to teach the procedures to others on the team, so cumulatively, we can be more efficient in the lab.
- Additionally, I have become much more adept at working as a team since joining the Sickle Cell Group. Collaborating on presentations and grant papers have allowed me to adapt these skills.
- I have always been a keen listener to others ideas, but I have realized to trust my own ideas more as well.
- I have become more effective in problem solving and problem framing as well. Finding a way towards approaching and solving complex problems is a key skill in the lab, while making presentations and more.
- Each member of the team has been assigned to present about what the project is about, lab protocols, purpose of our project, and/or protocols to external systems we use like ImageJ. We do this in our weekly meeting with Professor Cheng and our senior member Maria to ensure that everyone is on the same page and absorbing this information.
- All of our subgroups have to be on the same page to efficiently deliver results. For example, analyzing test line intensities, we need to make a lab schedule, go to the lab and run the experiment while following protocols, then send them to the ImageJ team for analysis.
- I have realized the importance of having background cultural knowledge. In high school, I modified a “Hippo Roller” to create electricity while being rolled to purify the water inside. I did not take the cultural aspects when prototyping the implementation of this device.
- Additionally, I have realized that to understand the true scope of our project, we need not only read previous articles from our team, but also previous articles regarding Sickle Cell Anemia and Lateral Flow Devices.
- I have also improved on my procrastination since joining the group. Many of my team members like to work ahead of schedule, so they have helped me be accountable for completing my work earlier.
2.
Roles:
Our team’s role is to successfully develop and administer a lateral flow immunoassay test strip in order to diagnose sickle cell disease (SCD) in Sierra Leone. The organizations and individuals (listed in “Relationships” below) identified the absolute need for a low-cost, screening device and have shown interest in what our solution proposes.
Relationships:
The project has gone through multiple stages of prototype designs to the design we have today with our PI, Professor Cheng, and GSIF coordinator, Khanjan Mehta. The team has also validated this device and has received feedback through the connections we have established with World Hope International, Sickle Cell Care Awareness Network (SCCAN) and Dr. Ranju Gupta together with partnerships within Sierra Leone including Masanga Hospital and Dr. Cheedy Jaja. We would also like to acknowledge Lehigh Global Social Impact Fellowship (GSIF), Lehigh Technical Entrepreneurship, Lehigh Valley Health Network and VentureWell E-team for bringing us this far along our work.
Procedures/Work in Progress as of Now:
So far, the team has a successful diagnostic test strip established. The novel device schematics, the “E-junction”, is capable of running on only a single drop of blood without a dilution step. The process is efficient and only takes 15 minutes to run. Currently, the diagnostic device is being tested using purified HbA and HbS, and also HbA blood samples from a local blood bank in Bethlehem. Our team has been awarded the Stage 1 grant of $5,000 offered by VentureWell E Team, the Davis Foundation’s Peace Prize, been selected as finalists for the Global Health track in the John Hopkins Healthcare Design Competition, invited to publish our year’s IEEE article onto a special issue indexed by SCOPUS called Multidisciplinary Sciences and Engineering organized by Advances in Science, Technology and Engineering Systems Journal (ASTESJ), received acceptance for this year’s IEEE GHTC Conference, and received acceptance for BMES 2020 Conference. We are currently under review for the NIH Tech Accelerator Challenge (NTAC) and DEBUT by VentureWell.
In the lab, we are currently running optimization tests to maximize the test line intensities. ImageJ software is used to quantify the test line intensities from these direct binding tests. Variations of the test line position on the test strip, as well as the location of one test line with respect to another test line, are being studied. Three test lines were printed with the HbA antibody on to the test strip at different locations relative to the absorbance pad. By doing this, we are seeing if there are possible indicators that inhibit or decrease the test line intensities. Test line intensities are conducted for the current prototype in order to measure for accuracy analysis with ImageJ software.
Current Goals and Milestones:
As our lateral flow device is improved and developed over time, our team plans to write publications. To do so, we are looking into journals to understand what our targeted audience wants to see from our research. For instance, in-depth concepts about our diagnostic device’s design, outcome, specificity, and sensitivity. Our experiments will be planned based on our target journal and two main focuses discussed in our publication will be design versus clinical tests versus field considerations. Some publications the team is considering as of right now are Lab on the Chip, and the Journal of Hematology.
Another goal the team is looking at is quality control. As a team, we must figure out the shelf-life and storage of the test strips (by itself or with the blood sample + running buffer + antibody). Possible storage we are looking into is the development of a casing. Other aspects to consider are also the effects of humidity, temperature and other possible constraints (dehydration).
After establishing quality control, we are currently working on optimizing the production of test strips. An automated repetitive system for the printing mechanism has been slowly developed alongside the diagnostic device. The system will consist of a conveyor belt and functionality programmed by an Arduino with Matlab. The goal is to create a motor-driven belt in which it could transport the strips of Nitrocellulose membranes down the conveyor. The Nitrocellulose membrane strips will reach a point, where it will trigger a light sensor (Photocell or Light Dependent Resistor Sensor), causing a chain reaction to enable an attached syringe press (propped up by a 3D printed stand) to pump the antibody solution onto the strip.
Next Steps and Larger Goals:
We aim to return to Sierra Leone in August of 2021 in order to conduct the alpha testing phase of the diagnostic device. To conduct this testing, we aim to collaborate with Dr. Jaja who has IRB approval to run studies on current SCD screening devices, SickleSCAN®. As we work under his guidance, an estimate of 10-20 devices will be tested to validate its usability.
We are planning to launch our product in Sierra Leone in Summer 2023. By cross-referencing a population density map and a map of all the public hospitals in Sierra Leone, we have constructed an ordered list of hospitals where we will introduce our device. One year after launch, we expect to be implemented in 10 densely populated hospitals encompassing many areas of the country. Two years after launch, we expect to be in 25 hospitals, and three years after launch, we expect to be in at least 50 hospitals diagnosing 75% of the newborns.
The device will be implemented into the standard operating procedures (SOP) of care in target hospitals where the hospital staff will issue the single-step test for SCD and Sickle Cell Trait (SCT) when a child is born.
Five years from launch, the venture will be sustainable and autonomous from our input as we will shift our focus to look at the intellectual side: strategic gaps, false positives/negatives. The project will be absorbed into the Sierra Leone Ministry of Health which will continue screening for SCD/SCT for newborns because they have the authority and funds to sustain our venture.
An impressive share, I just now with all this onto a colleague who had been doing a little analysis with this. And he the fact is bought me breakfast simply because I discovered it for him.. smile. So let me reword that: Thnx for the treat! But yeah Thnkx for spending some time to discuss this, I’m strongly regarding it and really like reading regarding this topic. When possible, as you become expertise, does one mind updating your website with more details? It can be highly great for me. Massive thumb up for this short article! customs house building
Thinking about updating your living space?
A home renovation Coogee can refresh your property and boost its value.
Skilled builders ensure quality results tailored to your style and needs.
Looking to give your home a fresh upgrade?
A home renovation Bondi can transform your property and add value.
Experienced builders deliver quality finishes tailored to your needs.
I found that site very usefull and this survey is very cirious, I ‘ ve never seen a blog that demand a survey for this actions, very curious… https://thegoldm.com/
Thank you so much for the post you do. I like your post and all you share with us is up to date and quite informative, i would like to bookmark the page so i can come here again to read you, as you have done a wonderful job. rc helicopter for adults
This post beautifully highlights the power of teamwork, perseverance, and growth in scientific research especially in developing meaningful solutions like the SCD diagnostic lateral flow device. Just like the Honey Don’t! Margaret Qualley Striped Shirt stands out with its bold, confident flair, your team’s dedication and innovation shine through every detail.
You’ll find it in addition a large set that we exceptionally savored researching. Will not be normal that come with the candidate to see or watch products. it şirkətləri
The post is written in very a good manner and it contains many useful information for me. เว็บตรง
Win88
79King
13Win
PG66 là nhà cái trực tuyến uy tín nhất tại thị trường Việt Nam, cung cấp hơn 1000+ tựa game slot, game bài, bắn cá, thể thao, tài xỉu,…..
Nice to read your article! I am looking forward to sharing your adventures and experiences. BINUSCX
Thank you for your own hard work on this website. Ellie enjoys conducting investigation and it is obvious why. All of us learn all of the compelling mode you produce simple tricks through this blog and as well cause participation from other ones about this area of interest then our daughter is now starting to learn a lot of things. Take advantage of the rest of the new year. You have been conducting a very good job. new build houses burnley
Wow, amazing blog format! How long have you ever been running a blog for? you make running a blog look easy. The overall glance of your web site is wonderful, as smartly the content material! 소액결제 현금화
This amazing is very much unquestionably great. All these smaller fact is manufactured working with number with credentials know-how. I actually enjoy the reasoning the best value. check up müayinə
Bu Dolandırıcıların Sitelerine Girmeyin: Çocuk Pornosu
Win88
888B
Thank you because you have been willing to share information with us. we will always appreciate all you have done here because I know you are very concerned with our. basket168
Well, the article is in reality the greatest on that worthw hile topic. I harmonise with your conclusions and also will certainly thirstily look forward to your approaching updates. Simply saying thanks will certainly not simply just be adequate, for the enormous lucidity in your writing. I can right away grab your rss feed to stay abreast of any updates. Admirable work and much success in your business enterprize! جهاز كشف الذهب
I am frequently to blogging and i also genuinely appreciate your content regularly. The content has really peaks my interest. I will bookmark your website and maintain checking for brand spanking new info. WhatsApp网页版
Here is the simplest and best assistance I have ever come across on this topic. Thank you for this very helpful blog post of yours. Additionally, I enjoy writing articles which has a personal tone incorporated. I feel it makes the reader feel more important along with inclined to trust me more. Plus it appears more real rather than coming from a robot. I quite like making my viewers feel important and also special. I want to hand them over the best tips on how to deal with important issues like this. home gym plans
Recently i found your site and so already reading along. I think overall We would leave my first comment. I don’t know very to say except that I’ve enjoyed reading. Nice blog. I would keep visiting this blog really often. free link indexing
98Win
Hi there, I discovered your site by way of Google whilst searching for a comparable topic, your
site got here up, it seems good. I’ve bookmarked
it in my google bookmarks.
Hi there, simply was aware of your blog thru
Google, and located that it is truly informative. I’m gonna be careful for brussels.
I’ll be grateful if you continue this in future. Numerous
other people will probably be benefited out of your writing.
Cheers!
Feel free to surf to my web blog … دریا زنده دل
I definitely enjoying every little bit of it. It is a great website and nice share. I want to thank you. Good job! You guys do a great blog, and have some great contents. Keep up the good work. anavar price
68Win
For88
Hırsız polis çağıracağım paramı geri verin Çocuk Pornosu
This is why it is far better that you simply applicable investigating well before building. You can actually design more effective share in such a manner. car background removal
My brother recommended I might like this website.
He was entirely right. This post truly made my day.
You can not imagine just how much time I had spent for this information! Thanks!
Here is my website :: Kelly himalaya hotsell replica hermes bags
SLOT88 menurut saya termasuk situs yang cukup fair, RTP-nya transparan dan gampang diakses.
Dari pengalaman main, sering dapat scatter maupun free spin. Cocok buat yang cari slot gacor harian.
Do you mind if I quote a couple of your articles as long as I provide credit and sources back to your website?
My blog is in the very same niche as yours and my
visitors would genuinely benefit from some of the information you provide
here. Please let me know if this okay with you.
Thanks!
Feel free to visit my website :: buôn bán nội tạng
Производство мебели из переработанного пластика.
Here is my web page … springfield university hospital glenburnie road london
Oopsmight have spoken too soon. Young teams dont know how to play late in games, especially playoff games. Total self-destruction. Can they now learn to win? Go Cs. 선릉쩜오
Good post. I certainly appreciate this website.
Stick with it!
Also visit my web-site :: lật đổ việt cộng
I absolutely love your blog.. Very nice colors & theme.
Did you make this site yourself? Please reply back as I’m attempting
to create my own personal blog and want to know where you got
this from or what the theme is called. Thank you!
Also visit my web-site; 789bet mobile
I really loved reading your blog. It was very well authored and easy to undertand. Unlike additional blogs I have read which are really not tht good. I also found your posts very interesting. In fact after reading, I had to go show it to my friend and he ejoyed it as well! WhatsApp网页版
I always rely on 오피가이드 for quality massage recommendations.
The post is written in very a good manner and it contains many useful information for me. เว็บหวย
I am happy to find your distinguished way of writing the post. Now you make it easy for me to understand and implement the concept. Thank you for the post. WhatsApp网页版
Hello, I have browsed most of your posts. This post is probably where I got the most useful information for my research. Thanks for posting, maybe we can see more on this. Are you aware of any other websites on this subject. 海外学位证
That is a very good viewpoint, however isn’t create any kind of sence at all preaching about that will mather. Just about any approach gives thanks and also thought about aim to reveal your current article in to delicius but it surely is apparently an issue using your sites is it possible please recheck this. many thanks once more. سكس
Great to see a resource tailored for non-technical business leaders https://promptswaale.online who still need to make critical AI-driven decisions.
Thanks for offering a well-organized collection of resources on your site. สมัคร หวย ลาว พัฒนา
I was suggested this blog via my cousin. I’m not sure whether this post is written by him as nobody else realize such particular about my trouble. You are incredible! Thank you! 0077 bet
With endless access to practice worksheets, OMT encourages sstudents tߋ master mathematics with repetition, constructing affection fоr thе
subject and exam confidence.
Transform math obstacles into victories ᴡith OMT Math Tuition’s mix of online ɑnd on-site choices, bacқed Ьy a track record ߋf trainee
quality.
Ρrovided that mathematics plays а pivotal function in Singapore’ѕ financial development аnd development, purchasing specialized math tuition equips students ԝith thｅ probⅼеm-solving
abilities neeԁed tо grow in а competitive landscape.
Enriching primary school education ᴡith math tuition prepares trainees
foг PSLE by cultivating ɑ development frɑmе ⲟf mind towaгd
tough topics like symmetry and improvements.
Offered tһe hiցh risks of Ⲟ Levels for senior һigh school development іn Singapore, math tuition makｅs the moѕt of opportunities fߋr top qualities and wanteⅾ placements.
Ιn an affordable Singaporean education ѕystem, junior college math
tuition оffers students the edge tߋ attain high qualities required fоr
university admissions.
OMT’ѕ custom-mɑⅾe math syllabus stands oᥙt by bridging
MOE content witһ sophisticated conceptual web lіnks,
helping trainees connect ideas аcross vari᧐ᥙs math
subjects.
Adult accessibility t᧐ progress reports ⲟne, enabling
support аt hоme for sustained grade renovation.
On-line math tuition ցives adaptability fоr active Singapore trainees,
enabling anytime accessibility tо resources fⲟr bettｅr exam preparation.
Ꮋere іs my homepage … secondary math tuition
주소은 안전한 주소를 검색하는 데 도움을 주는 서비스입니다. 사용자들은 다양한 분야의 링크를 편리하게 검색할 수 있으며, 이 주소들은 사용자들 간의 검증된 안전한 웹사이트들로 이루어져 있습니다. 따라서 주소나침반을 이용하는 것은 웹 서핑을 하면서 보다 신뢰할 수 있는 환경에서 활용할 수 있는 방법.
https://www.linkcompas.com/
Experience Singapore’ѕ favorite shopping portal at Kaizenaire.ｃom,curating а wide variety ⲟf promotions,
deals, ɑnd occasion specials from precious brand names.
Аs a busy shopping paradise, Singapore supplies tһe ideal playground foг citizens whо
adore promotions and brilliant deals.
Scuba diving journeys tо neighboring islands adventure
undersea explorers fгom Singapore, ɑnd bear in mind to remaіn updated
оn Singapore’ѕ latest promotions аnd shopping deals.
Matter Prints generates ethical textiles аnd clothes, valued Ьy sustainable shoppers іn Singapore
for their һand-block printed fabrics.
Gгeat Eastern ρrovides life insurance policy аnd health care prepares lor, beloved ƅy Singaporeans fⲟr their thoｒough insurance coverage ɑnd tranquility οf mind in uncertain tіmеs
leh.
Del Monte juices ԝith fruit cocktails аnd drinks,
treasured foг exotic, vitamin-rich alternatives.
Ԝhy wait one,get on Kaizenaire.сom foг deals sіɑ.
myweb site – loans comparison