Deterministic Lateral Displacement Cell Sorting

Deterministic lateral displacement (DLD) is a robust passive method that uses continuous flow in a microfluidic device for small-scale particle separation, such as beads, droplets, leukocytes, cancer cells, viruses, bacteria, and DNA. The particles separate after discharge through different shapes and sizes of geometry. Different modes are involved in this DLD system: displacement (bump) mode and zigzag mode, which could reflect the size difference of the sample. Regarding the free-form fabrication platform, we demonstrated a method to generate a series of multiplex designs in single device to sort distinct size particles and cells.